Carl read the papersWhat it actually is
TB-500 is a lab-made piece of Thymosin Beta-4 (TB4), a naturally-occurring protein found in nearly all cells. TB4 plays a role in building new blood vessels, cell migration and wound closure.
Where BPC-157 is often used near a specific injury, TB-500 is described as more systemic — spreading widely and acting body-wide, which is why the two are so often combined.
How it’s thought to work
- Actin regulation — binds actin, a protein central to cell movement and structure.
- Cell migration — helps repair cells travel to injury sites.
- Angiogenesis — supports new blood-vessel formation for blood flow.
- Anti-inflammatory — reported to reduce inflammation and scar tissue in models.
What the research says
Most data is pre-clinical and animal-based, with strong interest from veterinary (notably equine) medicine. Human trials are limited; much of the human use is anecdotal.
- Muscle, tendon and ligament recovery.
- Flexibility and reduced adhesions/scar tissue (reported).
- Wound healing and cardiovascular repair models.
Reported protocols
These are the doses the peptide community actually reports running (Reddit & forums), alongside published research — logged for education, not as a recommendation to use.
| Parameter | Commonly reported range |
|---|---|
| Community dose | 2–2.5 mg (loading); 2 mg (maintenance) |
| Frequency | Loading 2×/week ×4 wks → then 1×/week |
| Route | SubQ (some IM near injury) |
| Cycle | ~4–6 wk loading, then weekly (6–8+ wks total) |
| Community tip | Long half-life = infrequent dosing; refrigerate, use within ~28 days; run with BPC-157 |
Common use cases
| Goal | Community protocol notes |
|---|---|
| Systemic recovery | 2–2.5 mg 2×/wk loading, then 1×/wk |
| Flexibility / scar tissue | weekly maintenance, 6–8+ wks |
| With BPC-157 (Wolverine) | 2 mg TB 2×/wk + 250–500 mcg BPC daily |
Mixing it (reconstitution)
Freeze-dried peptides must be reconstituted with bacteriostatic water before they can be measured. Carl’s calculator turns “mg in the vial + ml of water + target dose” into “units on the syringe” — and tells you how long the vial lasts.
→ Open the Reconstitution Calculator
Injection & handling
SubQ (some IM near an injury), 2–2.5 mg — loading twice weekly for ~4 weeks, then weekly. The long half-life means infrequent dosing. Don’t blast BAC water onto the powder; refrigerate reconstituted, use within ~28 days.
Stacks it appears in
- The Wolverine — BPC-157 + TB-500 — local + systemic repair together.
